A Novel Route for Double-Layered Encapsulation of Streptomyces fulvissimus Uts22 by Alginate–Arabic Gum for Controlling of Pythium aphanidermatum in Cucumber

Damping-off disease due to Pythium aphanidermatum is one of the most harmful diseases cucumber. One critical issues in field biological control establishment a link between beneficial bacteria screened laboratory and its industrial application. Therefore, when developing biocontrol agents, it necessary study optimization mass production conditions select suitable carrier for their final formulation. In this study, an attempt was made provide formulation Streptomyces fulvissimus Uts22 strain based on alginate–Arabic gum nanoparticles (SiO2 TiO2) with layer-by-layer technique. X-ray diffraction (XRD) Fourier transform infrared spectroscopy (FTIR) studies showed that Arabic added composition, electrostatic interaction occurred alginate gum. The scanning electron microscope image beads show cubic shape good dispersion microcapsules. encapsulation efficiency prepared reported be 94%. maximum release from capsule recorded 35th day storage, about 109 CFU/gr. greenhouse experiments encapsulated resulted 95% reduction damping-off cucumber more potential effects increasing plant growth traits than free bacteria. This strategy can considered as alternative future applications agricultural field.